Trial: Lion's Mane (LC to Grain)

Progress Log

Start Date: Late May 2026

Method: Older LC syringes inoculated directly into sterilized grain jars.

Status: Success! Despite the age of the cultures, both jars have shown vigorous growth.

6-5-2026 (Update): Both jars are looking fantastic. Jar 1 is fully colonized and ready for a Grain to Grain (G2G) transfer to expand the culture. Jar 2 is just a few days behind and almost ready for its own G2G. This proves that even older LC can remain viable if stored properly.

Jar 1: Fully colonized and ready for G2G (6-05-2026).

Jar 2: Almost ready for G2G (6-05-2026).

6-10-2026 (Update): The G2G transfer from Jar 1 is already growing well and looking very healthy. The mycelium is jumping off the master grains and colonizing the new medium aggressively.

G2G transfer growing well and looking healthy (6-10-2026).

Next Steps

I will be performing G2G transfers from Jar 1 today to several new sterilized grain jars. Once Jar 2 is 100% colonized, it will also be used for expansion. The goal is to build up enough spawn for a large-scale fruiting trial on hardwood sawdust and soy hulls.