Trial: White Button

Cloning Agaricus bisporus from store-bought grocery specimens.

Progress Log

Start Date: 5-16-2026

Method: Core tissue sample moved to No-Pour Agar (Custom Corn Sugar & Yeast mix).

Quantity: 3 distinct plates.


5-21-2026: Mycelium is active, but surprisingly, this is currently the **slowest growing** of the three species. We are seeing clean white growth from all three samples, but it is taking its time to establish.

All White Button Plates

Group overview of the 3 White Button plates (Photo: 5-22-2026).

5-24-2026 (Update): Growth continues. Here is an overview of all 3 plates on Day 8.

All White Button Plates 2026-05-24

Group overview of the 3 White Button plates (Day 8).

6-1-2026 (Update): Steady progress. All three plates are showing healthy, clean white growth. They are still the slowest of the bunch, but they are consistently moving across the agar.

All White Button Plates 2026-06-01

Group overview of the 3 White Button plates (Day 16).

6-5-2026 (Update): Final check of the original clone plates. I chose the best-performing plate (Plate 1) and transferred it to 2 new agar plates for further expansion and cleanup.

All White Button Plates 2026-06-05

Final overview of clone plates before transfer (6-05-2026).

White Button New Transfers

New transfers from Plate 1 to two fresh agar plates (6-05-2026).

6-10-2026 (Update): The first generation of transfer plates is looking great. Both plates are showing healthy, clean growth with no signs of contamination. It's rewarding to see the culture establishing so well on the new agar.

White Button Transfer Progress

Healthy, clean growth on both transfer plates (6-10-2026).

6-16-2026 (Update): The transfers are progressing perfectly. Both plates look clean with good, healthy growth. This clone is now well-established on our custom agar mix.

White Button Transfers June 16

Vigorous, clean growth on the transfer plates (6-16-2026).

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