The Spores
Awakening the genetic potential. In my experience, working with spores must be done in as sterile an environment as possible to prevent contamination. I recommend using a Still Air Box (SAB), Oven Tek, or a Fan Filter Unit (FFU) if you have one. Minimizing air movement is what I've found to be critical to successful germination.
1. Spore Syringes (MSS)
My Golden Rule: Test on Agar First.
While it is tempting to inject directly into grain, I've found that Multi-Spore Syringes (MSS) are often not 100% sterile. Injecting directly into grain is a risk I prefer to avoid. Testing on agar first allows me to confirm the culture is clean and select healthy growth before I commit to a valuable grain jar.
How I use them: Shake the syringe vigorously to break up clumps. I've found you only need a tiny drop (0.1cc) in the center of an agar plate.
2. Spore Swabs
I find swabs are perfect for collecting spores directly from the gills of a mushroom. They can then be easily stored and shared.
Collection: I lightly rub between the gills. If I see spores on the swab, I've likely rubbed too hard and collected tissue. Spores are microscopic—if they are visible, there are already millions. I always let swabs dry in my sterile field (SAB/FFU) before repackaging.
Direct Streaking: I lightly "paint" or streak the swab across an agar plate in a zig-zag pattern. Note: I've found that once a swab touches agar, it cannot be safely stored for future use.
The Single-Strand Method: To preserve my library, I use flame-sterilized tweezers to pluck a single strand of cotton from the tip. I drag this strand across the agar or even slightly sub-surface. This makes it much easier to isolate monokaryons for breeding.
3. Spore Prints
I've found prints to be the most stable way to store genetics long-term. To make one, I place a fresh cap gills-down on aluminum foil and cover with a clean container for 12–24 hours.
Agar Scraping: I flame-sterilize a scalpel, let it cool, and lightly scrape a tiny amount of "spore dust" directly onto an agar plate.
My DIY Syringe: I also scrape a portion of a print into sterile water to make my own syringes. I let the syringe sit for 24 hours so the spores can rehydrate before I use them.
From Spore to Mycelium
Spores are a genetic shuffle—every germination is a new individual. In my kitchen, using agar is the only way to reliably select for the strongest, cleanest growth. When I see white, thread-like mycelium growing away from a dark spore spot, I know I'm on the right track. From here, I perform an A2A (Agar to Agar) transfer to a new plate to ensure I have a clean monoculture.